GenTegra RNAdvantage™

Whenever you have collected fresh tissue and would like to protect it, you have several options. The most common method always has been flash freezing, and the company GenTegra provided the product RNAlater to protect RNA at room temperature. Innovation, of course, never stops, and GenTegra has now introduced RNAdvantage, which offers significantly better RNA protection in tissues. With RIN scores that are over or at 1 better than RNAlater, and an overal RIN value of 7 or higher, RNAdvantage clearly is the next step in early RNA protection. You can be assured of excellent RNA sequencing results with these numbers. Your RNA extraction will never be rejected of too low RIN values.

The way of using RNAdvantage is identical to RNAlater, which means that it can be substituted easily without changes to your protocol. The benefits of RNAdvantage include rapid RNase inactivation, protection at room temperature for 7 – 14 days, no rtPCR or library prep inhibition, and it makes field collection of tissue samples much easier. The ability to store your samples at room temperature eliminates the need for power-consuming freezers, saving you money, but additionally lifting some of the burden that rests on our planet nowadays. Of course freezing is still an option, and it will extend the lifetime of your sample by an even greater amount.

The RIN values of protection of RNA by RNAdvantage has even exceeded the requirements at commercial sequencing services (Genewiz RIN ≥6.0 and Novogene RIN ≥5.8). The ratio of the 18s peak to the 28s peak is another metric for RNA integrity. Over an assortment of six different tissue types, the ratios for 28s/18s were consistently better for RNA tissue samples protected with RNAdvantage at 14 days then for RNAlater protected samples at 7 days. As an example, spleen tissue showed essentially no change between 7 days and 14 days at room temperature.

In every tissue tested by GenTegra the RIN score at 7-days for RNAdvantage exceeded the stringent requirement for a RIN score of ≥7 and in all tissues the 14-day RIN scores were such that excellent RNAseq could be performed on the samples. University core sequencing facilities often have more stringent requirements on the RIN number for RNA being submitted for RNAseq. The RIN values for the RNAdvantage protected samples are either at or very close to the Core lab requirements as to ensure excellent RNAseq results. Protect your RNA from the start in the best possible way.